Application of bio-priming to enhance osmotic stress tolerance in germinating coneflower (Echinacea purpurea (L.) Monch) seeds

Document Type : Original Article

Authors

1 Ph.D. candidate of department of Agronomy, Islamic Azad University of Karaj

2 Professors of Department of Agronomy and Plant Breeding, Karaj branch, Islamic Azad University, Karaj, Iran.

Abstract

Coneflower has antioxidative, antibacterial, antiviral, and antifungal properties, but it is sensitive to environmental stress. Osmotic stress is the main important environmental stress, especially in seed germination, and it is very important to provide environmentally friendly solutions to reduce the risks of this stress. For this purpose, a study was arranged as a factorial based on a completely randomized design with three replications on coneflower seeds. Treatments included four levels of bio-priming [control (no prime), Arbuscular mycorrhizal fungi extract, azotobacter (Azotobacter chroococcum), azospirillum (Azospirillum lipoferum)] as the first factor and four levels of osmotic stress [0 (control), -0.3 Mpa, -0.6 Mpa and -0.9] as the second factor. The results demonstrated that bio-priming application promoted the germination and growth characteristics of coneflower seedlings compared to control. Osmotic stress reduced germination indicators compared to control, bio-priming application alleviated the negative effect of osmotic stress. In control conditions (without osmotic stress), arbuscular mycorrhizal fungi extract (73.88%), azotobacter (73.74%) and azospirillum (72.31%) raised the germination percentage compared to control of bio-priming in -0.9 MPa of osmotic stress. Interaction of arbuscular mycorrhizal fungi extract and -0.9 MPa of osmotic stress (154.54%) and interaction of azotobacter and -0.9 MPa of osmotic stress (163.63%), increased catalase activity compared to control (without bio-priming and osmotic stress). Therefore, bio-priming is an effective environmentally friendly solution to alleviate the impact of osmotic stress on coneflower seedlings.

Keywords

References

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