Antioxidant and antibacterial investigation of metal nanoparticle extract of whole components (root, leaf and shoot mixture) of Actium lappa plant

Document Type : Original Article

Authors

1 Department of Chemistry, Roudehen Branch, Islamic Azad University, Roudehen, Iran

2 Young researchers and Elite Club, Science and Research Branch,Islamic Azad,University ,Tehran,Iran.

3 School of pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

In the past few years, many scientists and researchers have turned to the green synthesis method of nanoparticles from plant extracts in control and treatment of various cancers and infectious diseases. And there is no need to remove remaining organisms from synthesized materials. Then it is easier to control and inhibit growth of metal nanoparticles in extract. In this research, silver nanoparticles were synthesized by phytochemical method or green synthesis from Actium lappa plant extract. The characteristics of these silver nanoparticles were determined by TEM, DLS and UV-Vis absorption spectrophotometry. Obtained results from DPPH and ABTS methods showed strong and significant antioxidant effects (P≤0.05) for silver nanoparticles of this extract. As a result, the amount of inhibition of obtained free radicals from them was obtained at certain concentration of 0.72 mg/ml, equal to 86 and 71% respectively. And at high concentration, i.e. 1.4 mg/ml, free radical inhibition of artificial antioxidant (BHA) was obtained almost similar to this concentration. In diffusion disc method, the inhibition zone diameter increased with increase in concentration of silver nanoparticles of Actium lappa plant extract. And the values of MIC and MBC were obtained by this method.

Keywords

References

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