Free Radical Scavenging Activity and Total Phenol Content of Some Lamiaceae Family Species

Document Type : Original Article

Authors

1 Department of Horticultural Science, Maragheh Branch, Islamic Azad University, Maragheh, Iran

2 Department of Agronomy, Malakan Branch, Islamic Azad University, Malekan, Iran

3 Department of Horticultural science, Tabriz Branch, Islamic Azad University, Tabriz, Iran

Abstract

This study investigated the antioxidant activity and total phenol content of six species from the Lamiaceae family. We assessed the antioxidant capacity using two distinct methods: DPPH radical scavenging and ferric reducing antioxidant power (FRAP) assays. Peppermint (Mentha piperita L.) and Horsemint (Mentha longifolia (L.) Huds.) exhibited the strongest DPPH scavenging activity, with IC50 values of 100.7 ± 50.1 µg DW/mL and 80.9 ± 39.1 µg DW/mL, respectively. These species also demonstrated high FRAP values (49.9 ± 0.8 µM/g DW and 40.7 ± 1.8 µM/g DW, respectively), further confirming their robust reducing power. In contrast, Desert rod (Eremostachys laciniata (L.) Bunge), Thyme (Thymus deanensis Celak), and White horehound (Marrubium vulgare L.) displayed considerably lower antioxidant activity in both assays, indicating weaker radical scavenging and reducing capabilities. Salvia/Sage (Salvia hydrangea DC.) showed moderate antioxidant activity, falling between the highly active mint species and the less active group. Total phenol content, quantified as mg of catechin equivalents per g of dry weight (mg CE/g DW), was also determined. Notably, Horsemint possessed the highest total phenol content (20.9 ± 1.8 mg CE/g DW), aligning with its strong antioxidant activity. While a general trend was observed between higher total phenol content and increased antioxidant activity, this correlation was not absolute, suggesting the involvement of other antioxidant compounds or mechanisms. This comparative analysis highlights the diverse antioxidant potential within the Lamiaceae family and identifies Peppermint and Horsemint as promising sources of natural antioxidants, potentially valuable for applications in food, pharmaceutical, and cosmetic industries.

Keywords

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Journal of Organic Farming of Medicinal Plants
Volume 3, Issue 1
March 2024
Pages 1-6

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