In vitro evaluation of bio control agents and botanicals against mulberry root rot pathogen Fusarium oxysporum

Document Type : Original Article

Authors

1 Plant Pathology Department, Faculty of Agriculture, Islamic Azad University, Rasht Branch

2 Department of Plant protection, Ar.C., islamic Azad University, Arak, Iran

3 Member of the Agricultural Engineering System of Guilan Province

4 Graduated in Plant Pathology, Faculty of Agriculture, Islamic Azad University, Rasht Branch

5 Department of Agronomy And Plant Breeding, Arak Branch, Islamic Azad University, Arak, Iran

Abstract

Mulberry is only source of food for the silkworm (Bombyx mori) L. Mulberry is sensitive to soil borne diseases among them root rot is fast spreading disease caused by F. oxysporum. Pathogenes can be managed by synthetic chemical fungicides but the use of synthetic fungicides results in Toxic resedues on the growth and development of silkworms. In this study, the effect of antagonistic biological agents for the management of mulberry root rot pathogen was investigated. The extract of five herbs is used: garlic, peppermint, tea, thyme, and neem. The extracts were tested at three concentrations of 10, 10-1, 10-3 and three solvents of ethanol, methanol and water. The effect of the extracts in a certain concentration was investigated based on the method of mixing with the culture medium. Bacteria were purified from soil using serial dilution method and were examined based on dual culture, volatile compounds, extracellular extract, and antibiotics. The highest inhibition rate was observed in extracts obtained from ethanol solvent. Ethanolic extract of all plants except peppermint showed 100% inhibition. The least effect was observed for extracts dissolved in water. The highest inhibition rate water solvent was observed in neem extract with 58.34. In different solvents, the lowest inhibition rate was observed in extracts obtained from peppermint. 100 bacterial isolates were isolated from soil samples, of which five isolates showed antagonistic ability in various tests. The top bacteria were identified based on biochemical and molecular factors and belonged to the two genera Pseudomonas and Bacillus.

Keywords

References

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Journal of Organic Farming of Medicinal Plants
Volume 3, Issue 1
March 2024
Pages 44-49

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