Green Synthesis of Kelussia odoratissima Silver Nanoparticles: A Comprehensive Assessment of Potent Antibacterial and Antioxidant Activities

Document Type : Original Article

Authors

1 Department of Chemistry, Roudehen Branch, Islamic Azad University, Roudehen, Iran

2 Department of Environment, Ro.C. Islamic Azad University, Roudehen, Iran

3 Department of Chemistry, Ro.C. Islamic Azad University, Roudehen, Iran

Abstract

This study reports the green synthesis of silver nanoparticles using an aqueous extract of Kelussia odoratissima as a reducing and stabilizing agent. The formation of spherical, crystalline nanoparticles with an average size of 178±12 nm was confirmed by spectroscopic, electron microscopy, and X-ray diffraction analyses. A positive zeta potential (+22.4 mV) indicated high colloidal stability, while FTIR spectra confirmed the role of plant phenolic compounds in the reduction and capping process. Biological activity assessment demonstrated that the synthesized nanoparticles possess significant and concentration-dependent antibacterial effects against Gram-negative (e.g., Escherichia coli and Klebsiella pneumoniae) and Gram-positive (e.g., Staphylococcus aureus and Salmonella typhimurium) pathogens. The Minimum Inhibitory Concentration (MIC) was lowest for E. coli (19.8 ± 0.1 μg/mL) and highest for S. aureus (174 ± 1.2 μg/mL). The MBC/MIC ratio primarily indicated a bactericidal effect against the Gram-negative bacteria. They also showed significant antioxidant capacity in DPPH and ABTS assays. Overall, this work demonstrates the efficacy of K. odoratissima extract in producing biogenic silver nanoparticles with favorable biological properties.

Keywords

References

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